Effect
of Transfer Factor Advanced Formulas Containing
E-XF Blends on Natural Killer (NK) Cell
Activity
A
4Life Summary of an Independent NK Cell Study Report by
Calvin
W. McCausland, Ph.D. and Emma Oganova M.D., Ph.D.
Abstract
Summary
Objective
To
determine the extent to which Transfer Factor E-XF blends and
4Life Transfer Factor Plus Advanced Formula increases Natural
Killer (NK) cell activity above baseline.
Study
Design
The
blinded cytotoxicity study was designed by Dr.
E. Oganova and
Dr. C. McCausland. The independent testing of the coded samples
was done under the direction of Academician Anatoly Vorobiev,
M.D., Russian Academy of Medical Sciences (RAMS) and the experimental
work was conducted by. Dr. M.V. Kisielevsky, Dr. E.O. Khalturina,
at the Russian Cancer Research Center, RAMS.
Methods
Blood
was obtained from RORC donor station. A standard method of
density gradient was used to isolate mononuclear cells [NK
Cells]. The isolated cells, 60 thousand cells per well, in
100 ml of culture medium, were introduced into each well of
Costar 96 well plates. Then, portions of coded test samples
[Transfer
Factor E-XF blends of selected ratios, 4Life Transfer Factor
Plus E-XF blend and others samples] were introduced into the
wells at predetermined concentrations (wt/vol.). An Interleukin-2
(IL-2) standard was used to compare effectiveness. The
preparations were incubated in a COz-incubator with a 5% C02
atmosphere, 100% humidity and 37° C for 24 or 48 hours.
Next 30 thousand K-562 tumor cells (erythroblastic human leukemia)
were introduced into the wells. (Thus, the ratio of effector
and target cells was 2:1. Each sample was tested in triplicate).
The preparations containing both the effector (NK cells) and
target cells (K562 cancer cells) along with control wells were
again incubated for 18-24 hours under the same incubator conditions.
An MTT (dye) solution was used to spectro-photometricly determine
the number of viable cells remaining in each well. The cytotoxic
index (CI) expressed in % is reported for each sample.
Results
Each
of the samples significantly increased NK cell activity. The
Transfer Factor E-XF Blend (Advanced
Formula) resulted in greater NK cell activation (283%)
than the Transfer
Factor XF (204%). 4Life
Transfer Factor Plus Advanced Formula increased NK cell
activity by 437%. The
drug IL-2 increased NK cell active by 389%. In the study
activation by 4Life Transfer Factor Advanced Formula, 4Life
Transfer Factor Plus Advanced Formula and IL-2 resulted in
a kill rate of K562 cancer cells of 69%, 97% and 88% respectively
with the 48 hour incubation period giving the greatest NK cell
activation.
Discussion
As
previously reported in earlier publications two 4Life products,
4Life
Transfer Factor Classic and 4Life Transfer Factor Plus,
have been shown to significantly improve NK cell activation,
103% and 248% respectively. When the transfer factors from
patented egg technology are combined with bovine transfer factors
there is a synergistic activation ofNK cell activity. The improved
formulas, 4Life Transfer Factor and 4Life Transfer Factor Plus
Advanced Formulas improve NK cell activation by 283% and 437%
respectively.
4Life
researchers and scientists in developing the Advanced Formulas
were confident that the colostrum derived transfer factors
when combined with the egg
derived transfer factors (E-XF blends)
would enhance the activity and benefit of the products, but
we too were taken by surprise by the magnitude of the increase.
Further research needs to be done to better understand why
this synergism occurs. We are confident these results will
translate into increased benefits to consumers and increased
clinical effect in future studies.
This
study was conducted under the direction of Dr. Anatoli Vorobiev
by his colleagues of the Russian Academy of Medical Science.
Using established methods ofcytotoxicity testing, NK cells
from humans were combined with cancer cells and divided into
groups of transfer factors activated NK cells and groups ofunactivated
NK cells. The objective was to find the bovine and egg transfer
factor blend(s) (E-XF blends) that express the greatest NK
cell activation. The results enable us to identify the most
potent combination that will provide the greatest health benefits.
In
the control series of the experiments for all of the transfer
factor samples tested there was no direct cytotoxic effect
on tumor cells at any of the concentrations tested. When in
the absence of the immune NK cells the transfer factors are
combined directly with the cancer cells there is no cytotoxic
effect on the tumor cells.
Results
of this study clearly demonstrated that 4Life
Transfer Factor Advanced Formula and 4Life
Transfer Factor Plus Advanced Formula boosts NK cell activity. The independent research scientists
who conducted this study found the results interesting and
so exceptional as to request further information on the identity
of 4Life samples so that results could be published in professional
journals. Statements from researchers included the following:" The
4Life sample activated NK cell activity more than the Interleukin-2
(IL-2) drug used as the standard. Here, we now refer to
your sample as the Golden Interleukin," stated Dr. Kisielevsky,
Russian Academy of Medical Sciences.
We
strongly believe that these results are certain to be reflected
in improved human health resulting from significantly improved
immune response. It is our hope that people everywhere will
find that 4Life Transfer Factor Advanced Formula and 4Life
Transfer Factor Plus Advanced Formula will provide safe broad-spectrum
immune support that is unmatched in any other health supplement
today.
For
more information on any of 4Life’s™ products, please
feel free to contact us. Use your back button to return
to the homepage or newsletter that contains our contact information.
If you have any questions or need assistance with ordering,
we are pleased to help you in any way we can.
